mTagBFP2-Cx32-7
(Plasmid #55287)

• Purpose: Localization: Gap Junctions, Excitation: 399, Emission: 456
• Depositing Lab: Michael Davidson
• Publication: Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 Dec 8.

Backbone

• Vector backbone: mTagBFP2
• Backbone size w/o insert (bp): 4750
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cx32
• Alt name: Human Beta-1 Connexin 32
• Alt name: NM_001097642.2
• Species: H. sapiens (human)
• Entrez Gene: GJB1 (a.k.a. CMTX, CMTX1, CX32)
• Promoter: CMV
• Tag / Fusion Protein:
  • mTagBFP2 (C terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• Supplemental Documents:
  • mTagBFP2-Cx32-7 Data Supplement

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Industry MTA
  • Evrogen Ancillary Agreement for FP Distribution to Industry

Depositor Comments

H. sapiens (human) Beta-1 Connexin 32. Excitation = 399; Emission = 456