EBFP2-LaminB1-10
(Plasmid #55244)

• Purpose: Localization: Nuclear Envelope, Excitation: 383, Emission: 448
• Depositing Lab: Michael Davidson
• Publication: Davidson Blue Fluorescent Proteins (unpublished)

Backbone

• Vector backbone: EBFP2
• Backbone size w/o insert (bp): 4750
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: LaminB1
• Alt name: NM_005573.2
• Species: H. sapiens (human)
• Entrez Gene: LMNB1 (a.k.a. ADLD, LMN, LMN2, LMNB, MCPH26)
• Promoter: CMV
• Tag / Fusion Protein:
  • EBFP2 (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• Supplemental Documents:
  • EBFP2-LaminB1-10 Data Supplement
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

. Excitation = 383; Emission = 448