pFastBac His6 MBP Asn10 TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-C) Citations (16)
Originally described in: MacroBac: New Technologies for Robust and Efficient Large-Scale Production of Recombinant Multiprotein Complexes.Gradia SD, Ishida JP, Tsai MS, Jeans C, Tainer JA, Fuss JO Methods Enzymol. 2017;592:1-26. doi: 10.1016/bs.mie.2017.03.008. Epub 2017 May 15. PubMed Journal
Articles Citing pFastBac His6 MBP Asn10 TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-C)
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RNA-dependent chromatin association of transcription elongation factors and Pol II CTD kinases. Battaglia S, Lidschreiber M, Baejen C, Torkler P, Vos SM, Cramer P. Elife. 2017 May 24;6. pii: e25637. doi: 10.7554/eLife.25637. PubMed |
Nucleosome-Chd1 structure and implications for chromatin remodelling. Farnung L, Vos SM, Wigge C, Cramer P. Nature. 2017 Oct 26;550(7677):539-542. doi: 10.1038/nature24046. Epub 2017 Oct 11. PubMed |
Structures of transcription pre-initiation complex with TFIIH and Mediator. Schilbach S, Hantsche M, Tegunov D, Dienemann C, Wigge C, Urlaub H, Cramer P. Nature. 2017 Nov 9;551(7679):204-209. doi: 10.1038/nature24282. Epub 2017 Nov 1. PubMed |
Structure of activated transcription complex Pol II-DSIF-PAF-SPT6. Vos SM, Farnung L, Boehning M, Wigge C, Linden A, Urlaub H, Cramer P. Nature. 2018 Aug;560(7720):607-612. doi: 10.1038/s41586-018-0440-4. Epub 2018 Aug 22. PubMed |
Structural basis for acceptor RNA substrate selectivity of the 3' terminal uridylyl transferase Tailor. Kroupova A, Ivascu A, Reimao-Pinto MM, Ameres SL, Jinek M. Nucleic Acids Res. 2019 Jan 25;47(2):1030-1042. doi: 10.1093/nar/gky1164. PubMed |
Structural basis of TFIIH activation for nucleotide excision repair. Kokic G, Chernev A, Tegunov D, Dienemann C, Urlaub H, Cramer P. Nat Commun. 2019 Jun 28;10(1):2885. doi: 10.1038/s41467-019-10745-5. PubMed |
Structure of H3K36-methylated nucleosome-PWWP complex reveals multivalent cross-gyre binding. Wang H, Farnung L, Dienemann C, Cramer P. Nat Struct Mol Biol. 2020 Jan;27(1):8-13. doi: 10.1038/s41594-019-0345-4. Epub 2019 Dec 9. PubMed |
Structure of replicating SARS-CoV-2 polymerase.
Hillen HS, Kokic G, Farnung L, Dienemann C, Tegunov D, Cramer P.
Nature. 2020 May 21. pii: 10.1038/s41586-020-2368-8. doi: 10.1038/s41586-020-2368-8.
PubMed
Associated Plasmids |
Nucleosome-CHD4 chromatin remodeler structure maps human disease mutations. Farnung L, Ochmann M, Cramer P. Elife. 2020 Jun 16;9. pii: 56178. doi: 10.7554/eLife.56178. PubMed |
Structural basis of nucleosome transcription mediated by Chd1 and FACT. Farnung L, Ochmann M, Engeholm M, Cramer P. Nat Struct Mol Biol. 2021 Apr;28(4):382-387. doi: 10.1038/s41594-021-00578-6. Epub 2021 Apr 12. PubMed |
Structure of the human Mediator-RNA polymerase II pre-initiation complex. Rengachari S, Schilbach S, Aibara S, Dienemann C, Cramer P. Nature. 2021 Jun;594(7861):129-133. doi: 10.1038/s41586-021-03555-7. Epub 2021 Apr 26. PubMed |
Cryo-EM structure of mammalian RNA polymerase II in complex with human RPAP2. Fianu I, Dienemann C, Aibara S, Schilbach S, Cramer P. Commun Biol. 2021 May 21;4(1):606. doi: 10.1038/s42003-021-02088-z. PubMed |
Molecular architecture of the human tRNA ligase complex. Kroupova A, Ackle F, Asanovic I, Weitzer S, Boneberg FM, Faini M, Leitner A, Chui A, Aebersold R, Martinez J, Jinek M. Elife. 2021 Dec 2;10. pii: 71656. doi: 10.7554/eLife.71656. PubMed |
An examination of the metal ion content in the active sites of human endonucleases CPSF73 and INTS11. Huang J, Liu X, Sun Y, Li Z, Lin MH, Hamilton K, Mandel CR, Sandmeir F, Conti E, Oyala PH, Tong L. J Biol Chem. 2023 Apr;299(4):103047. doi: 10.1016/j.jbc.2023.103047. Epub 2023 Feb 22. PubMed |
Structural Basis of the Transcriptional Elongation Factor Paf1 Core Complex from Saccharomyces eubayanus. Qin Y, Zhou Y, Cao Y, Ren Y, Deng P, Jiang J, Wang Z. Int J Mol Sci. 2023 May 13;24(10):8730. doi: 10.3390/ijms24108730. PubMed |
Structural basis of Integrator-dependent RNA polymerase II termination. Fianu I, Ochmann M, Walshe JL, Dybkov O, Cruz JN, Urlaub H, Cramer P. Nature. 2024 May;629(8010):219-227. doi: 10.1038/s41586-024-07269-4. Epub 2024 Apr 3. PubMed |
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