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PurposeGUS-based microRNA target competitor used for testing function as target mimicry
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 55208 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGreen II 0229
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Backbone manufacturerpGreen II
- Backbone size w/o insert (bp) 2500
- Total vector size (bp) 6850
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Vector typePlant expression
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Selectable markersBasta
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namebeta-glucuronidase
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Alt nameGUS
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Insert Size (bp)1812
Resource Information
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A portion of this plasmid was derived from a plasmid made byModified from on pGreen II 0229
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGreen_GUS_competitor was a gift from Michael Axtell (Addgene plasmid # 55208 ; http://n2t.net/addgene:55208 ; RRID:Addgene_55208) -
For your References section:
Analysis of complementarity requirements for plant MicroRNA targeting using a Nicotiana benthamiana quantitative transient assay. Liu Q, Wang F, Axtell MJ. Plant Cell. 2014 Feb;26(2):741-53. doi: 10.1105/tpc.113.120972. Epub 2014 Feb 7. 10.1105/tpc.113.120972 PubMed 24510721