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pGreen_dualluc_ORF_sensor
(Plasmid #55207)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 55207 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGreen II
  • Backbone manufacturer
    pGreen II
  • Backbone size w/o insert (bp) 2500
  • Total vector size (bp) 7067
  • Vector type
    Luciferase ; Plant transient expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Renilla luciferase
  • Alt name
    R-Luc
  • Species
    Renilla reniformis
  • Insert Size (bp)
    936

Gene/Insert 2

  • Gene/Insert name
    Firefly luciferase
  • Alt name
    F-Luc
  • Species
    Photinus pyralis
  • Insert Size (bp)
    1653

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Modified from on pGreen II 0229 and pGreen II 0000
  • Articles Citing this Plasmid

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGreen_dualluc_ORF_sensor was a gift from Michael Axtell (Addgene plasmid # 55207 ; http://n2t.net/addgene:55207 ; RRID:Addgene_55207)

  • For your References section:

    Analysis of complementarity requirements for plant MicroRNA targeting using a Nicotiana benthamiana quantitative transient assay. Liu Q, Wang F, Axtell MJ. Plant Cell. 2014 Feb;26(2):741-53. doi: 10.1105/tpc.113.120972. Epub 2014 Feb 7. 10.1105/tpc.113.120972 PubMed 24510721
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