Pveg
(Plasmid #55173)

• Purpose: Very strong constitutive promoter
• Depositing Lab: Thorsten Mascher
• Publication: Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29.

Backbone

• Vector backbone: pSB1C3
• Backbone manufacturer: iGEM
• Backbone size w/o insert (bp): 2070
• Total vector size (bp): 2307
• Vector type: Synthetic Biology ; Bacillus BioBrick Box

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: veg promoter
• Alt name: Pveg
• Species: B. subtilis
• Insert Size (bp): 237
• Promoter: veg

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: PstI (not destroyed)
• 5′ sequencing primer: na

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Pveg is the strongest constitutive promoter in B. subtilis known to date.

For sequencing of insert, use the following primers:
fwd: TGCCACCTGACGTCTAAG
rev: ATTACCGCCTTTGAGTGA