mCherry-Vinculin-23
(Plasmid #55159)

• Purpose: Localization: Focal Adhesions, Excitation: 587, Emission: 610
• Depositing Lab: Michael Davidson
• Publication: Davidson lab - mCherry constructs (unpublished)

Backbone

• Vector backbone: mCherry
• Backbone size w/o insert (bp): 4750
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Vinculin
• Species: H. sapiens (human)
• GenBank ID: AAH39174.1
• Entrez Gene: VCL (a.k.a. CMD1W, CMH15, HEL114, MV, MVCL, VINC)
• Promoter: CMV
• Tag / Fusion Protein:
  • mCherry (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• Supplemental Documents:
  • mCherry-Vinculin-23 Data Supplement
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

. Excitation = 587; Emission = 610