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Addgene

mCherry-Tubulin-C-18
(Plasmid #55148)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 55148 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    mCherry-C1
  • Backbone size w/o insert (bp) 4750
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tubulin
  • Alt name
    NM_006082.2
  • Species
    H. sapiens (human)
  • Entrez Gene
    TUBA1B (a.k.a. K-ALPHA-1)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

. Excitation = 587; Emission = 610

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCherry-Tubulin-C-18 was a gift from Michael Davidson (Addgene plasmid # 55148 ; http://n2t.net/addgene:55148 ; RRID:Addgene_55148)

  • For your References section:

    Time-lapse two-color 3D imaging of live cells with doubled resolution using structured illumination. Fiolka R, Shao L, Rego EH, Davidson MW, Gustafsson MG. Proc Natl Acad Sci U S A. 2012 Apr 3;109(14):5311-5. doi: 10.1073/pnas.1119262109. Epub 2012 Mar 19. 10.1073/pnas.1119262109 PubMed 22431626
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