mCherry-Mito-7
(Plasmid #55102)

• Purpose: Localization: Mitochondria, Excitation: 587, Emission: 610
• Depositing Lab: Michael Davidson
• Publication: Olenych et al Curr Protoc Cell Biol. 2007 Sep;Chapter 21:Unit 21.5. doi: 10.1002/0471143030.cb2105s36.

Backbone

• Vector backbone: mCherry
• Backbone size w/o insert (bp): 4750
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Mito
• Alt name: NM_004074.2
• Species: H. sapiens (human)
• Entrez Gene: COX8A (a.k.a. COX, COX8, COX8-2, COX8L, MC4DN15, VIII, VIII-L)
• Promoter: CMV
• Tag / Fusion Protein:
  • mCherry (C terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• Supplemental Documents:
  • mCherry-Mito-7 Data Supplement
• Articles Citing this Plasmid:
  • 29 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

. Excitation = 587; Emission = 610