mCherry-miniSOG-Vinculin-23
(Plasmid #55100)

• Purpose: Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528
• Depositing Lab: Michael Davidson
• Publication: Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi: 10.1371/journal.pbio.1001041. Epub 2011 Apr 5.

Backbone

• Vector backbone: mCherry-miniSOG
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Vinculin
• Alt name: NM_003373.3
• Species: H. sapiens (human)
• Entrez Gene: VCL (a.k.a. CMD1W, CMH15, HEL114, MV, MVCL, VINC)
• Promoter: CMV
• Tags / Fusion Proteins:
  • mCherry (N terminal on backbone)
  • miniSOG (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Excitation = 448 / 473; Emission = 500 / 528

Please note that miniSOG is upstream of mCherry and Vinculin.