Skip to main content
Addgene

mCherry-H2B-6
(Plasmid #55056)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 55056 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    mCherry
  • Backbone size w/o insert (bp) 4750
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    H2B
  • Alt name
    Human Histone H2B
  • Alt name
    NM_021058.3
  • Species
    H. sapiens (human)
  • Mutation
    D26G and V119I in H2B
  • Entrez Gene
    H2BC11 (a.k.a. H2B/r, H2BFR, H2BJ, HIST1H2BJ)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

H. sapiens (human) Histone H2B. Excitation = 587; Emission = 610

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCherry-H2B-6 was a gift from Michael Davidson (Addgene plasmid # 55056 ; http://n2t.net/addgene:55056 ; RRID:Addgene_55056)

  • For your References section:

    Highly stable loading of Mcm proteins onto chromatin in living cells requires replication to unload. Kuipers MA, Stasevich TJ, Sasaki T, Wilson KA, Hazelwood KL, McNally JG, Davidson MW, Gilbert DM. J Cell Biol. 2011 Jan 10;192(1):29-41. doi: 10.1083/jcb.201007111. 10.1083/jcb.201007111 PubMed 21220507
Related items:
Commonly requested with: