mApple-Vinculin-23
(Plasmid
#54961)
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PurposeLocalization: Focal Adhesions, Excitation: 568, Emission: 592
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 54961 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonemApple
- Backbone size w/o insert (bp) 4750
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameVinculin
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Alt nameNM_003373.3
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SpeciesH. sapiens (human)
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MutationV234L in Vinculin.
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Entrez GeneVCL (a.k.a. CMD1W, CMH15, HEL114, MV, MVCL, VINC)
- Promoter CMV
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Tag
/ Fusion Protein
- mApple (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
. Excitation = 568; Emission = 592
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mApple-Vinculin-23 was a gift from Michael Davidson (Addgene plasmid # 54961 ; http://n2t.net/addgene:54961 ; RRID:Addgene_54961) -
For your References section:
Vinculin-actin interaction couples actin retrograde flow to focal adhesions, but is dispensable for focal adhesion growth. Thievessen I, Thompson PM, Berlemont S, Plevock KM, Plotnikov SV, Zemljic-Harpf A, Ross RS, Davidson MW, Danuser G, Campbell SL, Waterman CM. J Cell Biol. 2013 Jul 8;202(1):163-77. doi: 10.1083/jcb.201303129. 10.1083/jcb.201303129 PubMed 23836933