mApple-LAMP1-phLuorin-N-8
(Plasmid #54918)

• Purpose: Localization: Lysosome Membrane, Excitation: 568, Emission: 592
• Depositing Lab: Michael Davidson
• Publication: Davidson lab - mApple constructs (unpublished)

Backbone

• Vector backbone: mApple-N1
• Backbone size w/o insert (bp): 4750
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: LAMP1 phLuorin
• Alt name: NM_012857.2
• Species: R. norvegicus (rat)
• Mutation: Lysosomes-20 double labeled with phLuorin
• Promoter: CMV
• Tag / Fusion Protein:
  • mApple (C terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• Supplemental Documents:
  • mApple-LAMP1-phLuorin-N-8 Data Supplement
• Articles Citing this Plasmid:
  • 8 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Lysosomes-20 double labeled with phLuorin. Excitation = 568; Emission = 592

LAMP1 TM domain corresponds to aa 370-407 of LAMP1 (NM_012857.2).