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Purpose(Empty Backbone) Localization: Protein Expression Vector , Excitation: 513, Emission: 527
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Depositing Labs
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 54860 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backboneYpet-pBAD
- Backbone size (bp) 4800
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Vector typeBacterial Expression
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Tags
/ Fusion Proteins
- Ypet (C terminal on backbone)
- 6xHis (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tags
/ Fusion Proteins
- Ypet (C terminal on backbone)
- 6xHis (N terminal on backbone)
Resource Information
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A portion of this plasmid was derived from a plasmid made byP.S. Daugherty, PMID 15696158
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Contains 6x-Histidine tag for protein purification. Excitation = 513; Emission = 527
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
YPet-pBAD was a gift from Patrick Daugherty & Michael Davidson (Addgene plasmid # 54860 ; http://n2t.net/addgene:54860 ; RRID:Addgene_54860) -
For your References section:
Evolutionary optimization of fluorescent proteins for intracellular FRET. Nguyen AW, Daugherty PS. Nat Biotechnol. 2005 Mar . 23(3):355-60. 10.1038/nbt1066 PubMed 15696158