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Purpose(Empty Backbone) Localization: Protein Expression Vector , Excitation: 515, Emission: 528
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Depositing Labs
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 54859 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backboneVenus-pBAD
- Backbone size (bp) 4800
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Vector typeBacterial Expression
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Tags
/ Fusion Proteins
- Venus (C terminal on insert)
- 6xHis (N terminal on insert)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tags
/ Fusion Proteins
- Venus (C terminal on insert)
- 6xHis (N terminal on insert)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byA. Miyawaki, PMID 11753368
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Contains 6x-Histidine tag for protein purification. Excitation = 515; Emission = 528
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Venus-pBAD was a gift from Michael Davidson & Atsushi Miyawaki (Addgene plasmid # 54859 ; http://n2t.net/addgene:54859 ; RRID:Addgene_54859) -
For your References section:
A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nagai T, Ibata K, Park ES, Kubota M, Mikoshiba K, Miyawaki A. Nat Biotechnol. 2002 Jan;20(1):87-90. 10.1038/nbt0102-87 PubMed 11753368