Skip to main content
Addgene

tdTomato-pBAD
(Plasmid #54856)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 54856 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    tdTomato-pBAD
  • Backbone size (bp) 4800
  • Vector type
    Bacterial Expression
  • Tags / Fusion Proteins
    • tdTomato (C terminal on backbone)
    • 6xHis (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Tags / Fusion Proteins
    • tdTomato (C terminal on backbone)
    • 6xHis (N terminal on backbone)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    PMID 15558047; PI N.C. Shaner and R.Y. Tsien
  • Article Citing this Plasmid

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Contains 6x-Histidine tag for protein purification. Excitation = 554; Emission = 581

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    tdTomato-pBAD was a gift from Michael Davidson & Nathan Shaner & Roger Tsien (Addgene plasmid # 54856 ; http://n2t.net/addgene:54856 ; RRID:Addgene_54856)
  • For your References section:

    Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Shaner NC, Campbell RE, Steinbach PA, Giepmans BN, Palmer AE, Tsien RY. Nat Biotechnol. 2004 Dec;22(12):1567-72. Epub 2004 Nov 21. 10.1038/nbt1037 PubMed 15558047