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Purpose(Empty Backbone) Localization: Protein Expression Vector , Excitation: 554, Emission: 581
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 54856 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonetdTomato-pBAD
- Backbone size (bp) 4800
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Vector typeBacterial Expression
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Tags
/ Fusion Proteins
- tdTomato (C terminal on backbone)
- 6xHis (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tags
/ Fusion Proteins
- tdTomato (C terminal on backbone)
- 6xHis (N terminal on backbone)
Resource Information
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A portion of this plasmid was derived from a plasmid made byPMID 15558047; PI N.C. Shaner and R.Y. Tsien
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Contains 6x-Histidine tag for protein purification. Excitation = 554; Emission = 581
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
tdTomato-pBAD was a gift from Michael Davidson & Nathan Shaner & Roger Tsien (Addgene plasmid # 54856 ; http://n2t.net/addgene:54856 ; RRID:Addgene_54856) -
For your References section:
Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Shaner NC, Campbell RE, Steinbach PA, Giepmans BN, Palmer AE, Tsien RY. Nat Biotechnol. 2004 Dec;22(12):1567-72. Epub 2004 Nov 21. 10.1038/nbt1037 PubMed 15558047
