PS-mOrange2-N1
(Plasmid
#54849)
-
Purpose(Empty Backbone) Localization: N1 Cloning Vector, Excitation: 548 / 634, Emission: 565 / 662
-
Depositing Labs
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 54849 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonePS-mOrange2-N1
- Backbone size (bp) 4750
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
-
Tag
/ Fusion Protein
- PS-mOrange2 (C terminal on backbone)
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameNone
-
Tag
/ Fusion Protein
- PS-mOrange2 (C terminal on backbone)
Resource Information
-
A portion of this plasmid was derived from a plasmid made byV.V. Verkhusha, PMID 22900938
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
. Excitation = 548 / 634; Emission = 565 / 662
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
PS-mOrange2-N1 was a gift from Michael Davidson & Vladislav Verkhusha (Addgene plasmid # 54849 ; http://n2t.net/addgene:54849 ; RRID:Addgene_54849) -
For your References section:
A FRET-facilitated photoswitching using an orange fluorescent protein with the fast photoconversion kinetics. Subach OM, Entenberg D, Condeelis JS, Verkhusha VV. J Am Chem Soc. 2012 Aug 17. 10.1021/ja3034137 PubMed 22900938