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Purpose(Empty Backbone) Localization: Protein Expression Vector , Excitation: 530, Emission: 541
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Depositing Labs
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 54838 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonemPapaya-pBAD
- Backbone size (bp) 4800
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Vector typeBacterial Expression
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Tags
/ Fusion Proteins
- mPapaya (C terminal on backbone)
- 6xHis (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tags
/ Fusion Proteins
- mPapaya (C terminal on backbone)
- 6xHis (N terminal on backbone)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Contains 6x-Histidine tag for protein purification. Excitation = 530; Emission = 541
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mPapaya-pBAD was a gift from Robert Campbell & Michael Davidson (Addgene plasmid # 54838 ; http://n2t.net/addgene:54838 ; RRID:Addgene_54838) -
For your References section:
An engineered monomeric Zoanthus sp. yellow fluorescent protein. Hoi H, Howe ES, Ding Y, Zhang W, Baird MA, Sell BR, Allen JR, Davidson MW, Campbell RE. Chem Biol. 2013 Oct 24;20(10):1296-304. doi: 10.1016/j.chembiol.2013.08.008. Epub 2013 Oct 3. 10.1016/j.chembiol.2013.08.008 PubMed 24094838