mKate-pBAD
(Plasmid #54826)

• Purpose: (Empty Backbone) Localization: Protein Expression Vector , Excitation: 588, Emission: 635
• Depositing Labs: Dmitriy Chudakov, Michael Davidson
• Publication: Shcherbo et al Nat Methods. 2007 Sep . 4(9):741-6.

Backbone

• Vector backbone: mKate-pBAD
• Backbone size (bp): 4800
• Vector type: Bacterial Expression
• Tags / Fusion Proteins:
  • mKate (C terminal on backbone)
  • 6xHis (N terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Tags / Fusion Proteins:
  • mKate (C terminal on backbone)
  • 6xHis (N terminal on backbone)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: D.M. Chudakov, PMID 17721542
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Industry MTA
  • Evrogen Ancillary Agreement for FP Distribution to Industry

Depositor Comments

Contains 6x-Histidine tag for protein purification. Excitation = 588; Emission = 635