miniSOG-Lifeact-7
(Plasmid
#54823)
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Purpose(Empty Backbone) Localization: Actin, Excitation: 448 / 473, Emission: 500 / 528
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 54823 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backboneminiSOG-Lifeact
- Backbone size (bp) 4800
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
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Tags
/ Fusion Proteins
- miniSOG (C terminal on backbone)
- Lifeact (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- Lifeact (N terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Excitation = 448 / 473; Emission = 500 / 528. See Sequence Information page above for Addgene verified sequence.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
miniSOG-Lifeact-7 was a gift from Michael Davidson (Addgene plasmid # 54823 ; http://n2t.net/addgene:54823 ; RRID:Addgene_54823) -
For your References section:
A genetically encoded tag for correlated light and electron microscopy of intact cells, tissues, and organisms. Shu X, Lev-Ram V, Deerinck TJ, Qi Y, Ramko EB, Davidson MW, Jin Y, Ellisman MH, Tsien RY. PLoS Biol. 2011 Apr;9(4):e1001041. doi: 10.1371/journal.pbio.1001041. Epub 2011 Apr 5. 10.1371/journal.pbio.1001041 PubMed 21483721