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Addgene

miniSOG-Lifeact-7
(Plasmid #54823)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 54823 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    miniSOG-Lifeact
  • Backbone size (bp) 4800
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)
  • Tags / Fusion Proteins
    • miniSOG (C terminal on backbone)
    • Lifeact (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Tag / Fusion Protein
    • Lifeact (N terminal on backbone)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Excitation = 448 / 473; Emission = 500 / 528. See Sequence Information page above for Addgene verified sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    miniSOG-Lifeact-7 was a gift from Michael Davidson (Addgene plasmid # 54823 ; http://n2t.net/addgene:54823 ; RRID:Addgene_54823)

  • For your References section:

    A genetically encoded tag for correlated light and electron microscopy of intact cells, tissues, and organisms. Shu X, Lev-Ram V, Deerinck TJ, Qi Y, Ramko EB, Davidson MW, Jin Y, Ellisman MH, Tsien RY. PLoS Biol. 2011 Apr;9(4):e1001041. doi: 10.1371/journal.pbio.1001041. Epub 2011 Apr 5. 10.1371/journal.pbio.1001041 PubMed 21483721
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