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Purpose(Empty Backbone) Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581
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Depositing Labs
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 54813 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonemEos4b-Lifeact
- Backbone size (bp) 4800
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
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Tag
/ Fusion Protein
- mEos4b-Lifeact (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- mEos4b-Lifeact (C terminal on backbone)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
. Excitation = 505 / 569; Emission = 516 / 581
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mEos4b-Lifeact-7 was a gift from Michael Davidson & Loren Looger (Addgene plasmid # 54813 ; http://n2t.net/addgene:54813 ; RRID:Addgene_54813) -
For your References section:
Fixation-resistant photoactivatable fluorescent proteins for CLEM. Paez-Segala MG, Sun MG, Shtengel G, Viswanathan S, Baird MA, Macklin JJ, Patel R, Allen JR, Howe ES, Piszczek G, Hess HF, Davidson MW, Wang Y, Looger LL. Nat Methods. 2015 Jan 12. doi: 10.1038/nmeth.3225. 10.1038/nmeth.3225 PubMed 25581799