Kusabira Orange-N1 (KO)
(Plasmid
#54793)
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Purpose(Empty Backbone) Localization: N1 Cloning Vector, Excitation: 548, Emission: 561
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Depositing Labs
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 54793 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneKusabira Orange-N1
- Backbone size (bp) 4750
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
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Tag
/ Fusion Protein
- Kusabira Orange (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- Kusabira Orange (C terminal on backbone)
Resource Information
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A portion of this plasmid was derived from a plasmid made byA. Miyawaki; PMID 15065984
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Clontech MCS. Excitation = 548; Emission = 561
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Kusabira Orange-N1 (KO) was a gift from Michael Davidson & Atsushi Miyawaki (Addgene plasmid # 54793 ; http://n2t.net/addgene:54793 ; RRID:Addgene_54793) -
For your References section:
Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Karasawa S, Araki T, Nagai T, Mizuno H, Miyawaki A. Biochem J. 2004 Jul 1. 381(Pt 1):307-12. 10.1042/BJ20040321 PubMed 15065984
