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Purpose(Empty Backbone) Localization: N1 Cloning Vector, Excitation: 690, Emission: 713
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Depositing Labs
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 54787 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backboneiRFP-N1
- Backbone size (bp) 4750
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
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Tag
/ Fusion Protein
- iRFP (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- iRFP (C terminal on backbone)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Excitation = 690; Emission = 713.
Codon usage for iRFP is different than the original Nature Biotechnology paper (PMID 21765402).
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
iRFP-N1 was a gift from Michael Davidson & Vladislav Verkhusha (Addgene plasmid # 54787 ; http://n2t.net/addgene:54787 ; RRID:Addgene_54787) -
For your References section:
Bright and stable near-infrared fluorescent protein for in vivo imaging. Filonov GS, Piatkevich KD, Ting LM, Zhang J, Kim K, Verkhusha VV. Nat Biotechnol. 2011 Jul 17. doi: 10.1038/nbt.1918. 10.1038/nbt.1918 PubMed 21765402