IFP1.4-N1
(Plasmid
#54783)
-
Purpose(Empty Backbone) Localization: N1 Cloning Vector, Excitation: 684, Emission: 708
-
Depositing Labs
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 54783 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
* Log in to view industry pricing.
Backbone
-
Vector backboneIFP1.4-N1
- Backbone size (bp) 4750
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
-
Tag
/ Fusion Protein
- IFP1.4 (C terminal on backbone)
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameNone
- Promoter CMV
-
Tag
/ Fusion Protein
- IFP1.4 (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made byR.Y. Tsien, PMID 19423828
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Excitation = 684; Emission = 708
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
IFP1.4-N1 was a gift from Michael Davidson & Roger Tsien (Addgene plasmid # 54783 ; http://n2t.net/addgene:54783 ; RRID:Addgene_54783) -
For your References section:
Mammalian expression of infrared fluorescent proteins engineered from a bacterial phytochrome. Shu X, Royant A, Lin MZ, Aguilera TA, Lev-Ram V, Steinbach PA, Tsien RY. Science. 2009 May 8;324(5928):804-7. doi: 10.1126/science.1168683. 10.1126/science.1168683 PubMed 19423828
