Citrine-pBAD
(Plasmid
#54772)
-
Purpose(Empty Backbone) Localization: Protein Expression Vector , Excitation: 516, Emission: 529
-
Depositing Labs
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 54772 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
* Log in to view industry pricing.
Backbone
-
Vector backboneCitrine-pBAD
- Backbone size (bp) 4800
-
Vector typeBacterial Expression
-
Tags
/ Fusion Proteins
- Citrine (C terminal on insert)
- 6xHis (N terminal on insert)
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameNone
-
Tags
/ Fusion Proteins
- Citrine (C terminal on insert)
- 6xHis (N terminal on insert)
Resource Information
-
Supplemental Documents
-
A portion of this plasmid was derived from a plasmid made byR.E. Campbell, R.Y. Tsien, O. Griesbeck; PMID 11387331
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Contains 6x-Histidine tag for protein purification. Excitation = 516; Emission = 529
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
Citrine-pBAD was a gift from Robert Campbell & Michael Davidson & Oliver Griesbeck & Roger Tsien (Addgene plasmid # 54772 ; http://n2t.net/addgene:54772 ; RRID:Addgene_54772) -
For your References section:
Reducing the environmental sensitivity of yellow fluorescent protein. Mechanism and applications. Griesbeck O, Baird GS, Campbell RE, Zacharias DA, Tsien RY. J Biol Chem. 2001 Aug 3;276(31):29188-94. Epub 2001 May 31. 10.1074/jbc.M102815200 PubMed 11387331