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Addgene

mEGFP-N1
(Plasmid #54767)

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Item Catalog # Description Quantity Price (USD)
Plasmid 54767 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Cloning Grade DNA 54767-DNA.cg 2 µg of cloning grade DNA in Tris buffer 1 $105 *
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Backbone

  • Vector backbone
    mEGFP-N1
  • Backbone size (bp) 4750
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)
  • Tag / Fusion Protein
    • mEGFP (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Tag / Fusion Protein
    • mEGFP (C terminal on backbone)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A206K mutation. Excitation = 488; Emission = 507

Information for Cloning Grade DNA (Catalog # 54767-DNA.cg) ( Back to top)

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

  • Amount 2 µg
  • Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
  • Pricing $105 USD
  • Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).

Terms and Licenses

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mEGFP-N1 was a gift from Michael Davidson (Addgene plasmid # 54767 ; http://n2t.net/addgene:54767 ; RRID:Addgene_54767)
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