mWasabi-N1
(Plasmid #54765)

• Purpose: (Empty Backbone) Localization: N1 Cloning Vector, Excitation: 493, Emission: 509
• Depositing Labs: Robert Campbell, Michael Davidson
• Publication: Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/1741-7007-6-13.

Backbone

• Vector backbone: mWasabi-N1
• Backbone size (bp): 4750
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)
• Tag / Fusion Protein:
  • mWasabi (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Tag / Fusion Protein:
  • mWasabi (C terminal on backbone)

Resource Information

• Supplemental Documents:
  • mWasabi-N1 Data Supplement
• A portion of this plasmid was derived from a plasmid made by: R.E. Campbell and M.W. Davidson, PMID 18325109
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

mWasabi = mTFP1 + A66S-K139E-H163M-S216I. Excitation = 493; Emission = 509