mEGFP-C1
(Plasmid #54759)

• Purpose: (Empty Backbone) Localization: C1 Cloning Vector, Excitation: 488, Emission: 507
• Depositing Lab: Michael Davidson
• Publication: Michael Davidson Empty Backbones (unpublished)

Backbone

• Vector backbone: mEGFP-C1
• Backbone size (bp): 4750
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)
• Tag / Fusion Protein:
  • mEGFP (N terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Tag / Fusion Protein:
  • mEGFP (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• Supplemental Documents:
  • mEGFP-C1 Data Supplement
• Articles Citing this Plasmid:
  • 30 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

A206K mutation. Excitation = 488; Emission = 507. CMV-F and SV40pA-R primers are recommended. The EGFP-C primer is NOT recommended due to nonspecific binding.

Information for Cloning Grade DNA (Catalog # 54759-DNA.cg) ( Back to top)

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

• Amount: 2 µg
• Guaranteed Concentration: 100 ng/µl +/- 5 ng/µl
• Pricing: $105 USD
• Storage: DNA can be stored at 4℃ (short term) or -20℃ (long term).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here