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Purpose(Empty Backbone) Localization: C1 Cloning Vector, Excitation: 493, Emission: 509
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 54743 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonemWasabi-C1
- Backbone size (bp) 4750
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
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Tag
/ Fusion Protein
- mWasabi (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- mWasabi (N terminal on backbone)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byR.E. Campbell and M.W. Davidson, PMID 18325109
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
mWasabi = mTFP1 + A66S-K139E-H163M-S216I. Excitation = 493; Emission = 509
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mWasabi-C1 was a gift from Robert Campbell & Michael Davidson (Addgene plasmid # 54743 ; http://n2t.net/addgene:54743 ; RRID:Addgene_54743) -
For your References section:
Hue-shifted monomeric variants of Clavularia cyan fluorescent protein: identification of the molecular determinants of color and applications in fluorescence imaging. Ai HW, Olenych SG, Wong P, Davidson MW, Campbell RE. BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/1741-7007-6-13. 10.1186/1741-7007-6-13 PubMed 18325109
