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Purpose(Empty Backbone) Localization: N1 Cloning Vector, Excitation: 433, Emission: 475
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Depositing Labs
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 54742 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneCerulean-N1
- Backbone size (bp) 4750
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
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Tag
/ Fusion Protein
- Cerulean (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- Cerulean (C terminal on backbone)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byD.W. Piston, PMID 14990965
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
A206. Excitation = 433; Emission = 475
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Cerulean-N1 was a gift from Michael Davidson & Dave Piston (Addgene plasmid # 54742 ; http://n2t.net/addgene:54742 ; RRID:Addgene_54742) -
For your References section:
An improved cyan fluorescent protein variant useful for FRET. Rizzo MA, Springer GH, Granada B, Piston DW. Nat Biotechnol. 2004 Apr . 22(4):445-9. 10.1038/nbt945 PubMed 14990965
