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Addgene

Dronpa-Lifeact-7
(Plasmid #54740)

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Item Catalog # Description Quantity Price (USD)
Plasmid 54740 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    Dronpa-Lifeact
  • Backbone size (bp) 4800
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)
  • Tag / Fusion Protein
    • Dronpa-Lifeact (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Tag / Fusion Protein
    • Dronpa-Lifeact (C terminal on backbone)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

. Excitation = 503; Emission = 518

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Dronpa-Lifeact-7 was a gift from Michael Davidson (Addgene plasmid # 54740 ; http://n2t.net/addgene:54740 ; RRID:Addgene_54740)

  • For your References section:

    Nonlinear structured-illumination microscopy with a photoswitchable protein reveals cellular structures at 50-nm resolution. Rego EH, Shao L, Macklin JJ, Winoto L, Johansson GA, Kamps-Hughes N, Davidson MW, Gustafsson MG. Proc Natl Acad Sci U S A. 2012 Jan 17;109(3):E135-43. doi: 10.1073/pnas.1107547108. Epub 2011 Dec 12. 10.1073/pnas.1107547108 PubMed 22160683
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