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Purpose(Empty Backbone) Localization: N1 Cloning Vector, Excitation: 508 / 572, Emission: 518 / 580
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 54726 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneKaede-N1
- Backbone size (bp) 4750
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
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Tag
/ Fusion Protein
- Kaede (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- Kaede (C terminal on backbone)
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
. Excitation = 508 / 572; Emission = 518 / 580
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Kaede-N1 was a gift from Michael Davidson (Addgene plasmid # 54726 ; http://n2t.net/addgene:54726 ; RRID:Addgene_54726) -
For your References section:
Photoconversion in orange and red fluorescent proteins. Kremers GJ, Hazelwood KL, Murphy CS, Davidson MW, Piston DW. Nat Methods. 2009 May;6(5):355-8. doi: 10.1038/nmeth.1319. Epub 2009 Apr 12. 10.1038/nmeth.1319 PubMed 19363494