mCitrine-pBAD
(Plasmid #54723)

• Purpose: (Empty Backbone) Localization: Protein Expression Vector , Excitation: 516, Emission: 529
• Depositing Labs: Robert Campbell, Michael Davidson, Oliver Griesbeck, Roger Tsien
• Publication: Griesbeck et al J Biol Chem. 2001 Aug 3;276(31):29188-94. Epub 2001 May 31.

Backbone

• Vector backbone: mCitrine-pBAD
• Backbone size (bp): 4800
• Vector type: Bacterial Expression
• Tags / Fusion Proteins:
  • mCitrine (C terminal on backbone)
  • 6xHis (N terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Tags / Fusion Proteins:
  • mCitrine (C terminal on backbone)
  • 6xHis (N terminal on backbone)

Resource Information

• Supplemental Documents:
  • mCitrine-pBAD Data Supplement
• A portion of this plasmid was derived from a plasmid made by: R.E. Campbell, R.Y. Tsien, O. Griesbeck; PMID 11387331
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

Contains 6x-Histidine tag for protein purification. Excitation = 516; Emission = 529