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Purpose(Empty Backbone) Localization: Protein Expression Vector , Excitation: 600, Emission: 650
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Depositing Labs
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 54714 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonemNeptune-pBAD
- Backbone size (bp) 4800
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Vector typeBacterial Expression
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Tags
/ Fusion Proteins
- mNeptune (C terminal on backbone)
- 6xHis (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tags
/ Fusion Proteins
- mNeptune (C terminal on backbone)
- 6xHis (N terminal on backbone)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byN.C. Shaner, R.Y. Tsien, and R.E. Campbell, PMID 19942410
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Contains 6x-Histidine tag for protein purification. Excitation = 600; Emission = 650
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mNeptune-pBAD was a gift from Robert Campbell & Michael Davidson & Nathan Shaner & Roger Tsien (Addgene plasmid # 54714 ; http://n2t.net/addgene:54714 ; RRID:Addgene_54714) -
For your References section:
Autofluorescent proteins with excitation in the optical window for intravital imaging in mammals. Lin MZ, McKeown MR, Ng HL, Aguilera TA, Shaner NC, Campbell RE, Adams SR, Gross LA, Ma W, Alber T, Tsien RY. Chem Biol. 2009 Nov 25;16(11):1169-79. doi: 10.1016/j.chembiol.2009.10.009. 10.1016/j.chembiol.2009.10.009 PubMed 19942140