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Addgene

mKate1.31-Lifeact-7
(Plasmid #54668)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 54668 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

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Backbone

  • Vector backbone
    LifeAct-mKate1.31
  • Backbone size (bp) 4800
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)
  • Tag / Fusion Protein
    • LifeAct-mKate1.31 (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Tag / Fusion Protein
    • LifeAct-mKate1.31 (C terminal on backbone)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

mKate1.3 = mKate2. Excitation = 588; Emission = 635

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mKate1.31-Lifeact-7 was a gift from Michael Davidson (Addgene plasmid # 54668 ; http://n2t.net/addgene:54668 ; RRID:Addgene_54668)
  • For your References section:

    Far-red fluorescent tags for protein imaging in living tissues. Shcherbo D, Murphy CS, Ermakova GV, Solovieva EA, Chepurnykh TV, Shcheglov AS, Verkhusha VV, Pletnev VZ, Hazelwood KL, Roche PM, Lukyanov S, Zaraisky AG, Davidson MW, Chudakov DM. Biochem J. 2009 Mar 15;418(3):567-74. doi: 10.1042/BJ20081949. 10.1042/BJ20081949 PubMed 19143658