mRFP1-pBAD
(Plasmid #54667)

• Purpose: (Empty Backbone) Localization: Protein Expression Vector , Excitation: 584, Emission: 607
• Depositing Labs: Robert Campbell, Michael Davidson, Roger Tsien
• Publication: Campbell et al Proc Natl Acad Sci U S A. 2002 Jun 11;99(12):7877-82.

Backbone

• Vector backbone: mRFP1-pBAD
• Backbone size (bp): 4800
• Vector type: Bacterial Expression
• Tags / Fusion Proteins:
  • mRFP1 (C terminal on backbone)
  • 6xHis (N terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Tags / Fusion Proteins:
  • mRFP1 (C terminal on backbone)
  • 6xHis (N terminal on backbone)

Resource Information

• Supplemental Documents:
  • mRFP1-pBAD Data Supplement
• A portion of this plasmid was derived from a plasmid made by: R.E. Campbell and R.Y. Tsien; PMID 12060735
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Contains 6x-Histidine tag for protein purification. Excitation = 584; Emission = 607