-
Purpose(Empty Backbone) Localization: N1 Cloning Vector, Excitation: 574, Emission: 596
-
Depositing Labs
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 54644 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonemStrawberry-N1
- Backbone size (bp) 4750
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
-
Tag
/ Fusion Protein
- mStrawberry (C terminal on backbone)
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameNone
-
Tag
/ Fusion Protein
- mStrawberry (C terminal on backbone)
Resource Information
-
Supplemental Documents
-
A portion of this plasmid was derived from a plasmid made byPMID 15558047; PI N.C. Shaner and R.Y. Tsien
-
Articles Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
. Excitation = 574; Emission = 596
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
mStrawberry-N1 was a gift from Michael Davidson & Nathan Shaner & Roger Tsien (Addgene plasmid # 54644 ; http://n2t.net/addgene:54644 ; RRID:Addgene_54644) -
For your References section:
Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Shaner NC, Campbell RE, Steinbach PA, Giepmans BN, Palmer AE, Tsien RY. Nat Biotechnol. 2004 Dec;22(12):1567-72. Epub 2004 Nov 21. 10.1038/nbt1037 PubMed 15558047