CYPet-N1
(Plasmid
#54638)
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Purpose(Empty Backbone) Localization: N1 Cloning Vector, Excitation: 433, Emission: 475
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Depositing Labs
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 54638 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backboneCYPet-N1
- Backbone size (bp) 4750
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
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Tag
/ Fusion Protein
- CYPet (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- CYPet (C terminal on backbone)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byP.S. Daugherty, PMID 15696158
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
. Excitation = 433; Emission = 475
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CYPet-N1 was a gift from Patrick Daugherty & Michael Davidson (Addgene plasmid # 54638 ; http://n2t.net/addgene:54638 ; RRID:Addgene_54638) -
For your References section:
Evolutionary optimization of fluorescent proteins for intracellular FRET. Nguyen AW, Daugherty PS. Nat Biotechnol. 2005 Mar . 23(3):355-60. 10.1038/nbt1066 PubMed 15696158
