mOrange2-Peroxisomes-2
(Plasmid
#54596)
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Purpose(Empty Backbone) Localization: Peroxisomes, Excitation: 549, Emission: 565
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 54596 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonemOrange2
- Backbone size (bp) 4750
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
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Tags
/ Fusion Proteins
- mOrange2 (C terminal on backbone)
- Peroximal Targeting Signal 1 (PTS1) (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tags
/ Fusion Proteins
- mOrange2 (C terminal on backbone)
- Peroximal Targeting Signal 1 (PTS1) (C terminal on backbone)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
. Excitation = 549; Emission = 565
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mOrange2-Peroxisomes-2 was a gift from Michael Davidson (Addgene plasmid # 54596 ; http://n2t.net/addgene:54596 ; RRID:Addgene_54596) -
For your References section:
Fluorescent protein tracking and detection: fluorescent protein structure and color variants. Rizzo MA, Davidson MW, Piston DW. Cold Spring Harb Protoc. 2009 Dec;2009(12):pdb.top63. doi: 10.1101/pdb.top63. 10.1101/pdb.top63 PubMed 20150100