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Purpose(Empty Backbone) Localization: C1 Cloning Vector, Excitation: 516, Emission: 529
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Depositing Labs
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 54587 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonemCitrine-C1
- Backbone size (bp) 4750
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
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Tag
/ Fusion Protein
- mCitrine (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- mCitrine (N terminal on backbone)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byR.E. Campbell, R.Y. Tsien, O. Griesbeck; PMID 11387331
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Citrine + A206K. Excitation = 516; Emission = 529
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mCitrine-C1 was a gift from Robert Campbell & Michael Davidson & Oliver Griesbeck & Roger Tsien (Addgene plasmid # 54587 ; http://n2t.net/addgene:54587 ; RRID:Addgene_54587) -
For your References section:
Reducing the environmental sensitivity of yellow fluorescent protein. Mechanism and applications. Griesbeck O, Baird GS, Campbell RE, Zacharias DA, Tsien RY. J Biol Chem. 2001 Aug 3;276(31):29188-94. Epub 2001 May 31. 10.1074/jbc.M102815200 PubMed 11387331
