mTagBFP2-pBAD
(Plasmid #54572)

• Purpose: (Empty Backbone) Localization: Protein Expression Vector , Excitation: 399, Emission: 456
• Depositing Lab: Michael Davidson
• Publication: Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 Dec 8.

Backbone

• Vector backbone: mTagBFP2-pBAD
• Backbone size (bp): 4800
• Vector type: Bacterial Expression
• Tags / Fusion Proteins:
  • mTagBFP2 (C terminal on backbone)
  • 6xHis (N terminal on backbone)
  • T7 tag (N terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Tags / Fusion Proteins:
  • 6xHis (N terminal on backbone)
  • T7 tag (N terminal on backbone)

Resource Information

• Supplemental Documents:
  • mTagBFP2-pBAD Data Supplement
• Articles Citing this Plasmid:
  • 10 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Industry MTA
  • Evrogen Ancillary Agreement for FP Distribution to Industry

Depositor Comments

Contains 6x-Histidine tag for protein purification. Excitation = 399; Emission = 456

Information for Cloning Grade DNA (Catalog # 54572-DNA.cg) ( Back to top)

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

• Amount: 2 µg
• Guaranteed Concentration: 100 ng/µl +/- 5 ng/µl
• Pricing: $105 USD
• Storage: DNA can be stored at 4℃ (short term) or -20℃ (long term).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Industry MTA
  • Evrogen Ancillary Agreement for FP Distribution to Industry

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here