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Purpose(Empty Backbone) Localization: N1 Cloning Vector, Excitation: 399, Emission: 511
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Depositing Labs
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 54569 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonemT-Sapphire-N1
- Backbone size (bp) 4750
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
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Tag
/ Fusion Protein
- mT (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- mT (C terminal on backbone)
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Violet Excitation; EGFP + L64F-T65S-Q69M-C70V-S72A-Y145F-V163A-S175G-T203I-A206K. Excitation = 399; Emission = 511
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mT-Sapphire-N1 was a gift from Robert Campbell & Michael Davidson (Addgene plasmid # 54569 ; http://n2t.net/addgene:54569 ; RRID:Addgene_54569) -
For your References section:
Fluorescent protein FRET pairs for ratiometric imaging of dual biosensors. Ai HW, Hazelwood KL, Davidson MW, Campbell RE. Nat Methods. 2008 May . 5(5):401-3. 10.1038/nmeth.1207 PubMed 18425137
