MiCy-pBAD
(Plasmid #54565)

• Purpose: (Empty Backbone) Localization: Protein Expression Vector , Excitation: 472, Emission: 495
• Depositing Labs: Michael Davidson, Atsushi Miyawaki
• Publication: Karasawa et al Biochem J. 2004 Jul 1. 381(Pt 1):307-12.

Backbone

• Vector backbone: MiCy-pBAD
• Backbone size (bp): 4800
• Vector type: Bacterial Expression
• Tags / Fusion Proteins:
  • MiCy (C terminal on backbone)
  • 6xHis (N terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Tags / Fusion Proteins:
  • MiCy (C terminal on backbone)
  • 6xHis (N terminal on backbone)

Resource Information

• Supplemental Documents:
  • MiCy-pBAD Data Supplement
• A portion of this plasmid was derived from a plasmid made by: A. Miyawaki; PMID 15065984
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Contains 6x-Histidine tag for protein purification. Excitation = 472; Emission = 495