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Addgene

MiCy-pBAD
(Plasmid #54565)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 54565 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MiCy-pBAD
  • Backbone size (bp) 4800
  • Vector type
    Bacterial Expression
  • Tags / Fusion Proteins
    • MiCy (C terminal on backbone)
    • 6xHis (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Tags / Fusion Proteins
    • MiCy (C terminal on backbone)
    • 6xHis (N terminal on backbone)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Contains 6x-Histidine tag for protein purification. Excitation = 472; Emission = 495

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MiCy-pBAD was a gift from Michael Davidson & Atsushi Miyawaki (Addgene plasmid # 54565 ; http://n2t.net/addgene:54565 ; RRID:Addgene_54565)

  • For your References section:

    Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer. Karasawa S, Araki T, Nagai T, Mizuno H, Miyawaki A. Biochem J. 2004 Jul 1. 381(Pt 1):307-12. 10.1042/BJ20040321 PubMed 15065984
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