mPlum-pBAD
(Plasmid #54564)

• Purpose: (Empty Backbone) Localization: Protein Expression Vector , Excitation: 590, Emission: 649
• Depositing Labs: Michael Davidson, Roger Tsien
• Publication: Wang et al Proc Natl Acad Sci U S A. 2004 Nov 30;101(48):16745-9. Epub 2004 Nov 19.

Backbone

• Vector backbone: mPlum-pBAD
• Backbone size (bp): 4800
• Vector type: Bacterial Expression
• Tags / Fusion Proteins:
  • mPlum (C terminal on backbone)
  • 6xHis (N terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Tags / Fusion Proteins:
  • mPlum (C terminal on backbone)
  • 6xHis (N terminal on backbone)

Resource Information

• Supplemental Documents:
  • mPlum-pBAD Data Supplement
• A portion of this plasmid was derived from a plasmid made by: R.Y. Tsien; PMID 15556995
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Contains 6x-Histidine tag for protein purification. Excitation = 590; Emission = 649