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Purpose(Empty Backbone) Localization: Protein Expression Vector , Excitation: 551, Emission: 565
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 54555 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonemKO2-pBAD
- Backbone size (bp) 4800
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Vector typeBacterial Expression
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Tags
/ Fusion Proteins
- mKO2 (C terminal on backbone)
- 6xHis (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tags
/ Fusion Proteins
- mKO2 (C terminal on backbone)
- 6xHis (N terminal on backbone)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byPMID 18267078; PI A. Miyawaki
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Contains 6x-Histidine tag for protein purification. Excitation = 551; Emission = 565
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mKO2-pBAD was a gift from Michael Davidson & Atsushi Miyawaki (Addgene plasmid # 54555 ; http://n2t.net/addgene:54555 ; RRID:Addgene_54555) -
For your References section:
Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. Sakaue-Sawano A, Kurokawa H, Morimura T, Hanyu A, Hama H, Osawa H, Kashiwagi S, Fukami K, Miyata T, Miyoshi H, Imamura T, Ogawa M, Masai H, Miyawaki A. Cell. 2008 Feb 8;132(3):487-98. doi: 10.1016/j.cell.2007.12.033. 10.1016/j.cell.2007.12.033 PubMed 18267078
