mTFP1-pBAD
(Plasmid #54553)

• Purpose: (Empty Backbone) Localization: Protein Expression Vector , Excitation: 462, Emission: 492
• Depositing Labs: Robert Campbell, Michael Davidson
• Publication: Ai et al Biochem J. 2006 Dec 15. 400(3):531-40.

Backbone

• Vector backbone: mTFP1-pBAD
• Backbone size (bp): 4800
• Vector type: Bacterial Expression
• Tags / Fusion Proteins:
  • mTFP1 (C terminal on backbone)
  • 6xHis (N terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Tags / Fusion Proteins:
  • mTFP1 (C terminal on backbone)
  • 6xHis (N terminal on backbone)

Resource Information

• Supplemental Documents:
  • mTFP1-pBAD Data Supplement
• A portion of this plasmid was derived from a plasmid made by: R.E. Campbell; PMID 16859491
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Contains 6x-Histidine tag for protein purification. Excitation = 462; Emission = 492