-
Purpose(Empty Backbone) Localization: C1 Cloning Vector, Excitation: 507 / 572, Emission: 516 / 580
-
Depositing Labs
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 54550 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
* Log in to view industry pricing.
Backbone
-
Vector backbonemEos3.2-C1
- Backbone size (bp) 4750
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
-
Tag
/ Fusion Protein
- mEos3.2 (N terminal on backbone)
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameNone
-
Tag
/ Fusion Protein
- mEos3.2 (N terminal on backbone)
Resource Information
-
Supplemental Documents
-
A portion of this plasmid was derived from a plasmid made byT. Xu; PMID 22581370
-
Articles Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
mEos3.2 = mEos2 + I102N/H158E/Y189A. Excitation = 507 / 572; Emission = 516 / 580
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
mEos3.2-C1 was a gift from Michael Davidson & Tao Xu (Addgene plasmid # 54550 ; http://n2t.net/addgene:54550 ; RRID:Addgene_54550) -
For your References section:
Rational design of true monomeric and bright photoactivatable fluorescent proteins. Zhang M, Chang H, Zhang Y, Yu J, Wu L, Ji W, Chen J, Liu B, Lu J, Liu Y, Zhang J, Xu P, Xu T. Nat Methods. 2012 May 13;9(7):727-9. doi: 10.1038/nmeth.2021. 10.1038/nmeth.2021 PubMed 22581370
