mTagRFP-pBAD
(Plasmid #54539)

• Purpose: (Empty Backbone) Localization: Protein Expression Vector , Excitation: 555, Emission: 584
• Depositing Labs: Dmitriy Chudakov, Michael Davidson, Dorus Gadella
• Publication: Merzlyak et al Nat Methods. 2007 Jul;4(7):555-7. Epub 2007 Jun 17.

Backbone

• Vector backbone: mTagRFP-pBAD
• Backbone size (bp): 4800
• Vector type: Bacterial Expression
• Tags / Fusion Proteins:
  • mTagRFP (C terminal on backbone)
  • 6xHis (N terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Tags / Fusion Proteins:
  • mTagRFP (C terminal on backbone)
  • 6xHis (N terminal on backbone)

Resource Information

• Supplemental Documents:
  • mTagRFP-pBAD Data Supplement
• A portion of this plasmid was derived from a plasmid made by: D.M. Chudakov and T.W.J. Gadella; PMID 17572680

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Industry MTA
  • Evrogen Ancillary Agreement for FP Distribution to Industry

Depositor Comments

Contains 6x-Histidine tag for protein purification. Excitation = 555; Emission = 584