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Purpose(Empty Backbone) Localization: N1 Cloning Vector, Excitation: 505, Emission: 515. This plasmid encodes dClover2 A206K, H231L based on publication.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 54538 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonedClover2-N1
- Backbone size (bp) 4750
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
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Tag
/ Fusion Protein
- dClover2 (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
- Promoter CMV
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
. Excitation = 505; Emission = 515
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
dClover2-N1 was a gift from Michael Davidson (Addgene plasmid # 54538 ; http://n2t.net/addgene:54538 ; RRID:Addgene_54538) -
For your References section:
Improving brightness and photostability of green and red fluorescent proteins for live cell imaging and FRET reporting. Bajar BT, Wang ES, Lam AJ, Kim BB, Jacobs CL, Howe ES, Davidson MW, Lin MZ, Chu J. Sci Rep. 2016 Feb 16;6:20889. doi: 10.1038/srep20889. 10.1038/srep20889 PubMed 26879144
