mOrange2-pBAD
(Plasmid #54531)

• Purpose: (Empty Backbone) Localization: Protein Expression Vector , Excitation: 549, Emission: 565
• Depositing Labs: Michael Davidson, Nathan Shaner, Roger Tsien
• Publication: Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi: 10.1038/nmeth.1209. Epub 2008 May 4.

Backbone

• Vector backbone: mOrange2-pBAD
• Backbone size (bp): 4800
• Vector type: Bacterial Expression
• Tags / Fusion Proteins:
  • mOrange2 (C terminal on backbone)
  • 6xHis (N terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Tags / Fusion Proteins:
  • mOrange2 (C terminal on backbone)
  • 6xHis (N terminal on backbone)

Resource Information

• Supplemental Documents:
  • mOrange2-pBAD Data Supplement
• A portion of this plasmid was derived from a plasmid made by: N.C. Shaner, M.W. Davidson, and R.Y. Tsien; PMID 18454154
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Contains 6x-Histidine tag for protein purification. Excitation = 549; Emission = 565